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Image Search Results


Journal: eLife

Article Title: KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D

doi: 10.7554/eLife.44478

Figure Lengend Snippet:

Article Snippet: VEGFR-3 and VEGFR-2 were precipitated from the cell lysate using Strep-Tactin Sepharose (IBA, Göttingen, Germany) for strep-tagged VEGFR-2/–3 or immunoprecipitated using protein A Sepharose (PAS) and anti-VEGFR-3 (clone 9D9F9, ) or anti-VEGFR-2 (AF357, R and D Systems), washed three times with PBS/0.05% Tween-20/1 mM Na 3 VO 4 and eluted with 2x Laemmli buffer and analyzed by SDS-PAGE/Western blot using the phospho-tyrosine-specific antibody 4G10 (Merck/Millipore, Darmstadt, Germany, 1:5000).

Techniques: Expressing, Transfection, Construct, Mutagenesis, Recombinant, Virus, Positive Control, Negative Control, Transformation Assay, Clinical Proteomics, Neutralization, Enzyme-linked Immunosorbent Assay, Plasmid Preparation